17 November
Au-Minecraft
A new approach towards the impedimetric biosensing system
Au-Minecraft
A new approach towards the impedimetric biosensing system
The Au-Minecraft is the system that will allow for more precise electrochemical detection of selected biochemical interactions of proteins, enzymes, and DNA. This task will be possible thanks to the innovative use of gold nanostructures, appropriately surface-modified to identify host-guest interactions. The project will examine and determine how changes in the self-organization of gold nanostructures will affect charge transfer in the diffusion areas. The Au-Minecraft will detect impedance parameters resulting from changes in the heterogeneity level at the electrode surface, caused by the occurrence of specific intermolecular interactions and intentional, controlled disturbance of the sensor surface geometry. The project was financed by the National Science Centre in OPUS call (2020/37/B/ST7/03262).
Team
Members
Prof. Jacek Ryl
Principal Investigator - GUTAssociate Professor at Institute of Nanotechnology and Materials Engineering, GUT. Materials engineer and applied electrochemistry specialist, dedicated to impedimetric studies of the biosensing nanosystems, signal processing, and physicochemical studies.
Dr Paweł Niedziałkowski
Principal Investigator - UGAssistant Professor at Department of Analytical Chemistry, UG. An analytical and organic chemist, within project responsible for Au nanocubes synthesis and surface functionalization procedures as well as electrochemical studies.
Prof. Bożena Nejman-Faleńczyk
InvestigatorAssociate Professor at Department of Molecular Biology, UG, focused on small non-coding RNA molecules in the development of phages in bacterial cells. In project dedicated to preparation target biochemical interaction protocols at the Au-Minecraft system.
Prof. Robert Bogdanowicz
InvestigatorAssociate Professor at Department of Metrology and Optoelectronics, GUT. Materials engineer and optoelectronic specialist, responsible for the design of the Au-Minecraft formation processes, optoelectric studies of the nanostructures.
Dr Sylwia Bloch
InvestigatorAssistant Professor in the Laboratory of Phage Therapy, IBB PAS. Her main research interests are focused on phage biodiversity, regulation of processes occurring during phage development. In project dedicated to studying receptor/co-receptor interactions
Prof. Tadeusz Ossowski
Senior ExpertFull Professor and Head of the Department of Analytical Chemistry, UG. An expert in analytical chemistry, applied electrochemistry, and physical chemistry. Research interests include organic synthesis, thermodynamics, and kinetics at interfaces.
Prof. Artur Zieliński
InvestigatorAssociate Professor at Faculty of Chemistry, GUT. Electrochemist and nanoimaging expert. His role is determination of model procedures for charge transfer kinetics and its implementation as well as measurements of local surface electric heterogeneities.
Prof. Grzegorz Węgrzyn
Senior ExpertFull Professor and Head of the Department of Molecular Biology, UG. His scientific works focus on mechanisms of DNA replication regulation and gene expression in bacterial cells and diagnostics, and new methods of treating human genetic diseases.
Mr Adrian Koterwa
PhD student - scholarPhD student at Department of Analytical Chemistry, UG. Specialized in analytical chemistry and electrochemistry, responsible for the synthesis of Au nanocubes and electrochemical research of functionalized surfaces.
Miss Karolina Sapiega
MSc student - scholarM.Sc. student of biomedical engineering at GUT. Investigating electrochemistry of gold nanoarchitecture and gold-TiO2 heterojunction for biosensing applications, particularly with transparent transducers.
Mr Adrian Olejnik
PhD student - scholarPhD student at Nanodiamond Team, GUT. Materials engineer and electrochemistry specialist. Responsible for computational simulations of receptors and their interactions with Au-Minecraft, modeling biochemical interactions and electric signal transfer.
Mr Mateusz Brodowski
PhD student - scholarPh.D. student at GUT Faculty of Applied Physics and Mathematics and member of GUT Nanodiamond Team. Specialist in electrochemistry and electrode functionalization for biosensing. Responsible for investigation of charge transfer by Au nanocube structures
Miss Małgorzata Nadolska
PhD student - scholarPh.D. student at GUT Institute of Nanotechnology and Materials Engineering. Specialist in surface analysis, in particular spectroscopy techniques and adsorption phenomena. Dedicated to solid surfaces functionalization and immunoassays evaluation.
Miss Klaudia Prusik
MSc student - scholarM.Sc. student of Materials Engineering at GUT, specializing in photoactive materials and photocatalysis. Investigating AuNC heterojunctions and combined, opto-electrochemical (bio)sensor action
Project
News
Public
Resources
2021-01-08
Jacek Ryl, Pawel Slepski
6th International Symposium on Surface Imaging/Spectroscopy at the Solid/Liquid Interface
A new approach towards the impedance biosensing by multifrequency measurements
In this conference presentation, Jacek discussed how the utilization of Dynamic Electrochemical Impedance Spectroscopy in potentiodynamic or galvanostatic mode can be used for biosensing purposes, including flow injection tests and studies at charged up electrode surfaces surface heterogeneity changes, use for receptor evaluation, and more.
2020-07-27
Paweł Niedziałkowski, Paweł Ślepski, Joanna Wysocka, Joanna Chamier-Ciemińska, Łukasz Burczyk, Michał Sobaszek, Anna Wcisło, Tadeusz Ossowski, Robert Bogdanowicz, Jacek Ryl
Sensors and Actuators B: Chemical
Multisine impedimetric probing of biocatalytic reactions for label-free detection of DEFB1 gene: How to verify that your dog is not human?
In this work, we utilized for the very first time the DEIS for electrochemical sensing. The target of our study was the DEFB1 gene, which is unique to the human species. The analysis in a wide polarization range allowed us to find significant differences in the electric signal if DNA hybridization took place at the electrode surface, marking successful detection. Confirmation of the possibility to control frequency dispersion of capacitance in biosensing systems was the groundwork for our Au-Minecraft project.
Au-Minecraft
Contact details
Address
Narutowicza 11/12 str., Gdansk Gdansk University of Technology Faculty of Applied Physics and MathematicsHead of Project
Prof. Jacek Ryl, D.Sc. Ph.D.Contact
jacek.ryl@pg.edu.pl +48 58 348 66 06Universities
